DaphTox II

Biomonitoring using Daphnia

Sensitive detection of toxic substances in water via computer-assisted digital image analysis

The bbe DaphTox II observes daphnids, commonly known as "water fleas", under the influence of constantly running sample water. Based on the Extended Dynamic Daphnia Test, bbe developed a new sensitive method to detect hazardous compounds in water from rivers (source-water protection), plants, distribution systems and production drains to preserve human health and to monitor water.

Sensitivity data are available below.

Sample water (0.5 - 2 l/h) continuously runs through the measuring chamber containing the daphnia. The live images obtained using a CCD-camera are evaluated online with an integrated PC to analyse changes in the behaviour of the daphnia. If the change is statistically significant, an alarm is triggered. The method of image analysis enables a series of measurement methods and plausibility tests to assess the daphnia's behaviour using different criteria.

Measurement using digital image analysis
Observation of speed parameters:

  • average speed
  • speed distribution
  • distance between daphnia

Observation of behaviour:

  • swimming height
  • location
  • fractal dimension: measurements for turns and circling movements, curviness

Observation of growth:

  • determination of daphnia size

Toxicity Index
The concept of the toxicity index is based on the evaluation of certain measurands, such as speed or height, and changes in these measurands. Only when 2 or more of the measurands simultaneously show unusual results within a fixed period of time does the DaphTox II trigger an alarm.

Features & Informations

Applications

Surface water applications

  • dam monitoring
  • waterway analysis and assessment
  • general environmental monitoring

Drinking water applications

  • intake assessment
  • drinking water supply

Research applications

  • risk analysis
  • limnological work
  • research and education

Features

Chamber and camera

  • 1-chamber system with up to 10 daphnids or other organisms
  • optional 2-chamber system with enhanced reliability
  • easily removable daphnia chamber - prevents air bubbles and increases detection rate

Sample preparation

  • integrated sample treatment (microfiltration, ultrasonic cleaning and pre-heating)
  • air-cooled peltier element - no separate cooling water

Low maintenance, few consumables

  • simple operation:
    15" touchscreen PC with splash-proof flexible USB keyboard (supplied), Windows XP software
  • automatic, prepared concentrated algae feeder syringe - no algae culture necessary
  • fewer tube connections

Data transfer options

  • LAN
  • Modem
  • Relays
  • 4-20 mA
  • USB
  • ModBus

Software

The database software is used to record and analyse the data.
The most important features are...

  • easy data analysis and management
  • new upper and lower threshold values for all relevant measurands
  • parameterisation of the measurements
  • saving of data and parameters in a database
  • graphic display of measurement values
  • data export to Excel or text files
  • two protected user levels
  • print function

Technical specification of our DaphTox II

Description Value
Toxicity detection due to video analysis of daphnia behaviour with regard to the following:
Measurands
  • average swim speed
  • speed distribution
  • swim height
  • average distance
  • fractal dimension of paths: curviness, turns
  • no. of daphnia
  • distribution in chamber
  • size of daphnia
Measurement procedure video image analysis
Housing material varnished steel plate
Weight 60 kg
Dimensions (H x W x D) 800 x 800 x 500 m
Protection class IP54
Mains supply 110/240V 50/60Hz
Power consumption 600 W
Temperature Sample: 0 - 30 °C / Environment: 0 - 35° C
Transmission suitable for max. 100 FTU
Sample volume 30 ml
Maintenance interval > 7 days
Sample inflow free inflow/tube pump
Hardware integrated touchscreen PC, Windows XP Prof. splash-proof, flexible USB keyboard
Outputs
  • modem
  • LAN
  • analogue output 4-20 mA (2 X) (optional)
  • relay output (2 X)
  • RS232
  • USB

Subject to alteration!

Available variants of the DaphTox II

Daphnia Toximeter 115V/60Hz, 1-chamber system

Itemnumber: BG10700

The Daphnia Toximeter is used for the determination of toxic compounds in water and is conceived as part of an Early Warning System (EWS) to continuously monitor water pollution. The toximeter is a stand-alone unit and is able to exchange information with an external control system.

Instrument construction:

  • 1 chamber with up to 10 organisms each
  • sieve compartment to made from solvent- and corrosion-resistant materia

Compact housing:

  • protection class IP54
  • forced drain
  • collector sump
  • separate compartments for flow-through cell and electric components
  • 15" touchscreen PC, LAN, modem and USB connections
  • instrument operation and parameter set-up

Instrument supervision:

  • hardware control with sensor-monitored malfunction analysis

Temperature control:

  • conditioning of sample water pre-treatment with micro-filtration, ultrasound cleaning and pre-heating unit
Request a quote

Daphnia Toximeter 115V/60Hz, 2-chamber, 1-channel system

Itemnumber: BG10800

The Daphnia Toximeter is used for the determination of toxic compounds in water and is conceived as part of an Early Warning System (EWS) to continuously monitor water pollution. The toximeter is a stand-alone unit and is able to exchange information with an external control system.

Instrument construction:

  • 2 chambers with up to 10 organisms each, sample passes through each chamber in series
  • sieve compartment to made from solvent- and corrosion-resistant material

Compact housing:

  • protection class IP54
  • forced drain
  • collector sump
  • separate compartments for flow-through cell and electric components
  • 15" touchscreen PC, LAN, modem and USB connections
  • instrument operation and parameter set-up

Instrument supervision:

  • hardware control with sensor-monitored malfunction analysis

Temperature control:

  • conditioning of sample water pre-treatment with microfiltration, ultrasound cleaning and pre-heating
Request a quote

Daphnia Toximeter 230V, 1-chamber system

Itemnumber: BG10500

The Daphnia Toximeter is used for the determination of toxic compounds in water and is conceived as part of an Early Warning System (EWS) to continuously monitor water pollution. The toximeter is a stand-alone unit and is able to exchange information with an external control system.

Instrument construction:

  • 1 chamber with up to 10 organisms each
  • sieve compartment to made from solvent- and corrosion-resistant materia

Compact housing:

  • protection class IP54
  • forced drain
  • collector sump
  • separate compartments for flow-through cell and electric components
  • 15" touchscreen PC, LAN, modem and USB connections
  • instrument operation and parameter set-up

Instrument supervision:

  • hardware control with sensor-monitored malfunction analysis

Temperature control:

  • conditioning of sample water pre-treatment with microfiltration, ultrasound cleaning and pre-heating unit
Request a quote

Daphnia Toximeter 230V, 2-chamber system

Itemnumber: BG10600

The Daphnia Toximeter is used for the determination of toxic compounds in water and is conceived as part of an Early Warning System (EWS) to continuously monitor water pollution. The toximeter is a stand-alone unit and is able to exchange information with an external control system.

Instrument construction:

  • 2 chambers with up to 10 organisms each, sample passes through each chamber in series
  • sieve compartment to made from solvent- and corrosion-resistant material

Compact housing:

  • protection class IP54
  • forced drain
  • collector sump
  • separate compartments for flow-through cell and electric components
  • 15" touchscreen PC, LAN, modem and USB connections
  • instrument operation and parameter set-up

Instrument supervision:

  • hardware control with sensor-monitored malfunction analysis

Temperature control:

  • conditioning of sample water pre-treatment with microfiltration, ultrasound cleaning and pre-heating
Request a quote

Downloads & Information about the DaphTox II

Manuals and further informations for this product may be available in the internal area for registered users.